Isolation of nuclear proteins from human brains.
نویسندگان
چکیده
chain electrophoresis: a new approach to the determination of the GgAg ratio in fetal haemoglobin and to studies of globin synthesis. Br. J. Haematol. 44:527-534. 2.Dionisi, H.M., S.K. Checa and A.M. Viale. 1995. Protein immunoblotting of stained gels. BioTechniques 19:348-349. 3.Harrington, M.G. 1990. Elution of proteins from gels, p. 488-495. In M.P. Deutscher (Ed.), Methods in Enzymology, Vol. 182. Academic Press, New York. 4.Harwig, S., N. Chen, A. Park and R. Lehrer. 1993. Purification of cysteine-rich bioactive peptides from leukocytes by continuous acidurea-polyacrylamide gel electrophoresis. Anal. Biochem. 208:382-386. 5.Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680685. 6.Little, J.W. 1991. Low-tech method for removing Coomassie blue from polyacrylamide gels. BioTechniques 11:759. 7.Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275. 8.Merril, C.R., D. Goldman and M.L. Van Keuren. 1984. Gel protein stains: silver stains, p. 441-447. In J.E. Celis and R. Bravo (Eds.), Methods in Enzymology, Vol. 104. Academic Press, New York. 9.Molnar, A., P. Kulcsár and G.D. Prestwich. 1990. Effective removal of excess Coomassie blue from polyacrylamide gels. BioTechniques 9:534-536. 10.Vanfleteren, J.R., J.G. Raymackers, S.M. Van Bun and L.A. Meheus. 1992. Peptide mapping and microsequencing of proteins separated by SDS-PAGE after limited in situ acid hydrolysis. BioTechniques 12:550-557.
منابع مشابه
Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis
Ornithobacterium rhinotracheale (ORT) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. Four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in Mazandaran province. These birds were suspected to pasteurellosis by the farm veterinarian. No other marked gross lesion except ema...
متن کاملSerum Factors Induced the Nuclear Location of Annexin V in the Human Osteosarcoma Cell Line (MG-63)
Calcium-binding proteins play essential roles in the cell. One important class of calcium-binding proteins is the annexin family. This is a family of 13 proteins, which binds to phospholipids in a calcium-dependent manner. Osteosarcoma cell line (MG-63) is a transformed cell that has many characteristics of the differentiated cell, such as a considerable serum dependency in its growth rate. Usi...
متن کاملIsolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...
متن کاملAnatomical Variations of Circle of Willis in 57 Human Brains
Purpose: The circle of willis is responsible for blood supply of brain, which may suffer from anatomical variations. These disturbances can lead to aneurysm or even arterial rupture. The purpose of this study was to evaluate the anatomical structure of the cerebral arteries forming the circle of willis in adult cadavers. Also, cerebral versus non- cerebral versus non-cerebral arterial walls wer...
متن کاملIsolation and identification of excretory-secretory and somatic antigens from the Oestrus ovis larvae by SDS-PAGE and immunoblotting
Oestrus ovis is an economically important parasite of small ruminants and a zoonotic parasite with many reports of ophthalmomyiasis in human from Iran and other countries. The aim of the peresent studywas the isolation and identification of excretory-secretory (ES) and somatic (S) antigens of O. ovis second and third stage larvae (L2, L3) collected from Arabi sheep breeds loca...
متن کاملSexual Dimorphism in Volume of Precentral Cortex of Human Brain in Normal Subject who are Suffering from Alzheimer and Parkinson-A Stereological and Macroscopical Study
Purpose: This study is designed to determine the sex difference in volume of precentral cortex of left hemisphere in right-handed normal subjects and the right-handed subjects which are suffering from Alzheimer and Parkinson diseases. Materials and Methods: This study was performed on 72 normal human brains (38 males, 34 females), 11 human brains suffering from Alzheimer (4 males, 7 females), ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- BioTechniques
دوره 22 5 شماره
صفحات -
تاریخ انتشار 1997